Composite

Part:BBa_K2443032

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)


RRF optimized for expression in E. coli


Ribosome Recycling Factor (RRF) is responsible for recycling ribosomes after completion of protein synthesis. it has been Codon optimized for use in Escherichia coli and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of RRF is 22 kDa, which closely corresponds to the band in lane 9 of the overexpression gel below. Protein was overexpressed from BL21DE3 gold containing pJET vector with the RRF coding sequence. Protein overexpression was performed according to the protocol on the Lethbridge 2017 iGEM wiki, and notebook (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf)

Lethbridge_Oct_22_gel.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/cell-free_tx-tl
Parameters
None